Table 1.
Transformation and editing frequencies of reagents targeting the PHO2-B and PHO2-C haplotypes.
| Reagent | Explants | Regenerated plants | NPTII +ve | TF (%) | T0 plants screened | Edited T0 plants | Edited (%) | 1ko | 2ko | 3ko | 4ko | Avg size deletion |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| pDIRECT: PHO2-B/C: 6xplex: Csy4 | 144 | 110 | 67 | 46.5 | 12 | 8 | 66.7 | 5 | 2 | 1 | 0 | 7-bp |
| pTRANS_220: PHO2-B/C: 6xplex: Csy4: TREX2 | 144 | 107 | 93 | 64.6 | 21 | 18 | 85.7 | 8 | 6 | 4 | 0 | 64-bp |
| pTRANS_220: PHO2-B/C: 6xplex: tRNA: TREX2 | 144 | 144 | 115 | 79.9 | 18 | 12 | 66.7 | 6 | 2 | 2 | 2 | 191-bp |
“NPTII+ ve” denotes regenerated shoots that were found positive forone or more T-DNA copies by PCR analysis. The “TF (%)” indicates transformation efficiency calculated by the number of NPTII +ve plants divided by the number starting explants. The “Edited (%)” was calculated by dividing the number T0 edited plants by the number screened T0 plants. The “1–4ko” indicates the number of individual haplotype knock-outs per plant.