Fig. 1.

Meiotic synchronization allows for robust stage-specific isolation of mRNA. a) A schematic outlining 2 parallel meiotic time courses using the indicated inducible genetic systems that enhance cell synchrony. Arrows indicate when mRNA was harvested and samples with color-matched arrows were combined to produce mRNA libraries representing 5 meiotic stages. b) Heat map displaying relative mRNA expression profiles from (a) ordered by hierarchical clustering. Two replicates for each time course are shown and the color-matched labels for replicates 1 and 2 indicate the cDNA library that each row represents. Columns represent the normalized TPM of each gene across each library and replicate.