Fig. 2.

Gene representation in meiotic cDNA libraries. a) A schematic of inducible yeast cDNA library construction. TGIRT-III reverse transcriptase was used for cDNA synthesis and Gateway cloning was used for plasmid cloning. Red dots represent the GAL1/10 promoter. Plasmids were transformed into yeast using electroporation. b) DNA-seq was used to estimate the number of genes represented in the indicated libraries. The Venn diagram shows cDNAs that are unique or common between the 5 libraries. c) GSEA of all stages of library construction for the “early meiosis” library vs the other 4 cDNA libraries (“Rest”). Vertical black bars represent positioning of genes in the “DNA replication and recombination” clusters from Brar et al. (2012). The heat map indicates genes that are more enriched in the “early meiosis” libraries (red, left-side) or more enriched in the other libraries (blue, right-side). NES, normalized enrichment score. d) GSEA analysis as in (c), except all stages of library construction for the “meiosis I” library is being compared with the other 4 cDNA libraries (“Rest”). Vertical black bars represent positioning of genes in the Ndt80 cluster from Cheng et al. (2018). The heat map indicates genes that are more enriched in the “meiosis I” libraries (red, left-side) or genes that are more enriched in the other libraries (blue, right-side). NES, normalized enrichment score.