Fig. 4.

Assessing the effect of meiotic gene expression on competitive fitness. a) Schematic of competitive fitness assay. Each meiotic library was grown in uninducing (UN) or inducing (IND) conditions for 5 days. Cultures were grown to saturation each day, a sample was taken for DNA-seq, and the culture was rediluted 1 in 1,000 for another day of growth (∼8 divisions per day). b) Monotonicity Z-scores (MZ-scores) were calculated for every gene in uninducing (UN) and inducing (IND) conditions and the cumulative distribution is shown (left plot). Zoomed-in plots for data above and below the dotted lines are shown for the individual libraries on the right. Gray boxes highlight MZ-score of −1.8 (top row) and 1.8 (bottom row), which is the cutoff for genes considered to be disenriched or enriched over time, respectively. P-values signify whether induction of the cDNA library causes a detectable change in the number of genes becoming disenriched (top row) or enriched (bottom row). c) The number of genes that are disenriched (red) or enriched (yellow) in uninducing (UN) or inducing (IND) conditions. d) Normalized levels of genes that disenrich (red) or enrich (yellow) over the course of 5 days are plotted for uninducing (top row) or inducing (bottom row) conditions.