Fig. 6.

Overexpression of mitochondrial proteins is detrimental to competitive fitness and disrupts respiration. a) Strains overexpressing the indicated genes and untagged PGK1 were grown in competition with an equal number of cells containing an empty vector (pUB914) and PGK1-GFP. The percentage of GFP+ cells was measured by flow cytometry and plotted after 1 and 2 days of growth in inducing media. Data for 3 biological replicates are shown. b) Strains overexpressing the indicated genes and PGK1-GFP were grown in competition with an equal number of cells containing an empty vector (pUB914) and untagged PGK1. The percentage of GFP+ cells was measured by flow cytometry and plotted after 1 and 2 days of growth in inducing media. Data for 3 biological replicates are shown. c) Strains overexpressing the indicated gene were recovered from the freezer in media containing raffinose (uninducing) overnight and then diluted into media containing glucose (uninducing) for growth measurements over 24 h (Day 1-Uninduced). Each growth curve represents the average of 3 biological replicates. d) Growth curves as in (c) except cells were recovered from the freezer in media containing raffinose (uninducing) overnight and then diluted into media containing galactose (inducing) for growth measurements over 24 h (Day 1-Induced). Each growth curve represents the average of 3 biological replicates. e) After 24 h of growth, cells from (d) were rediluted into media containing galactose (inducing) for growth measurements over 48 h (Day 2-Induced). Each growth curve represents the average of 3 biological replicates. f) Cells from (d) were rediluted into media containing glycerol for growth measurements over 48 h (Day 2-Glycerol). Each growth curve represents the average of 3 biological replicates.