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. 2022 Mar 28;12(6):jkac070. doi: 10.1093/g3journal/jkac070

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© The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Performance of selected terminators in vitro. a) Diagram of transcription templates for in vitro testing using recombinant T7RNAP. The 3 numbers underneath each diagram show the transcription initiation site, the predicted site of termination, and the length of the run-off product. Hairpin symbols represent the cloned T7 terminators. b) In vitro termination efficiency of selected terminators. The error bars represent standard deviation from the mean. Different letters denote statistically significant differences (P < 0.05) according to a Tukey multiple comparison test.