Fig. 3.

In vivo performance of selected terminators with the bacterial polymerase EcRNAP. a) Terminator sequences were placed between fluorescent protein-encoding genes located downstream of the lac promoter, using BamHI and KpnI restriction sites. Plasmids were transformed into E. coli MG1655 and reporter gene expression was used to assess terminator efficiency. b) Bar chart of the mean termination efficiency of engineered terminators for EcRNAP. Error bars represent standard deviation from the mean. Different letters denote statistically significant differences (P < 0.05) according to a Tukey multiple comparison test.