Figure 5.
Quantitative microbiome analysis shows bacterial abundance increase and diversity decrease after fly eclosion
(A) No obvious difference in the OTU relative abundance between the age-related surface microbiomes at the bacterial phylum level.
(B) Variation of the OTU relative abundance between the age-related surface microbiomes at the bacterial genus level.
(C) Age-dependent increase of bacterial abundance on flies. The same color fragments represent the same genus of bacteria in panels B and C.
(D) Venn diagram analysis of the OTUs shared between different DPE flies.
(E) Substantial decrease of the observed OTU numbers (Sobs) in an age-dependent manner.
(F) Substantial decrease of the Shannon H index in an age-dependent manner. Panels E and F: Data are represented as mean ± SD. The 2-DPE flies were used as a reference for the two-tailed Student’s t test of difference: ∗∗∗, p < 0.001; ∗∗∗∗, p < 0.0001. There were eight independent replicates for each treatment. (See also Figures S4 and S5).