Figure 3.

Evolved isolates from different time points show increased productivity on the root relative to the ancestor

The ancestor and evolved isolates from populations 3, 4, 6, and 7 at three different time points (T12, 18, 30) were tested for individual root colonization. For each evolved isolate, relative root colonization was calculated by dividing the log10-transformed productivity (CFU/mm root) of each replicate by the mean of the log10-transformed productivity of the ancestor from the same experimental setup. The cross represents the mean relative root colonization (N = 3-4). The dashed, horizontal line represents the mean of the ancestor (N = 3-4), whereas the grey-shaded rectangles represent the SD of the ancestor from the corresponding experiment. The normalized values were subjected to a One-sample t-test to test whether the mean was significantly different from 1. p-values have been corrected for multiple comparisons. ∗p < 0.05, ∗∗p < 0.01. See also Figure S2.