Figure 5.
Evolved isolates from T30 show altered pellicle biofilm formation in response to plant polysaccharides and impaired motility
(A) Ancestor and evolved isolates from transfer 30 were inoculated into LB + 0.5% xylan at a starting OD600 of 0.05 in 24-well plates. Images were acquired after 48 h incubation at 30°C using a stereomicroscope. Each image is representative of four replicates. Each well has 16 mm width. Evolved isolates were tested for swimming (B) and swarming (C) motility in LB medium supplemented with 0.3 or 0.7% agar, respectively.
(B) Bars represent the mean (N = 3-4) and error bars represent SD.
(C) Lines represent the mean (N = 2-4) and error bars the SD. For the motility assays, the following statistical analysis applies: For each time point, an ANOVA was performed on the log10-transformed data followed by a Dunnett’s Multiple Comparison test with the ancestor as the control. For swarming motility, the asterisks show the least significance observed for the given time point. At 3 h, only isolate 7.3 was significantly reduced in swarming motility. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. See also Tables S1, S2, Figures S3, S4, and S5.