Figure 2.

Characterization of cancer-cell-culture-derived EVs

(A) Transmission electron microscopy (TEM) image of EVs released from prostate cancer cells.

(B) TEM of isolated EVs.

(C) Canonical markers immunogold (6nm)-labeled CD81 shows specificity to the vesicle surface. The scale bar is 100nm.

(D) Immunofluorescence co-localization analyses of EVs confirmed the presence of canonical tetraspanins on the vesicle surface. Mouse IgG antibody yielded a minimum signal.

(E) Optical image of immunofluorescence co-localization analyses shows EVs specificity to CD9 but not to mouse IgG.

(F) Nanoparticle tracking analysis (NTA) shows size and concentration of isolated EVs.

(G) Zeta potential of EVs shows moderate colloidal stability between the EVs and their surrounding fluid environment.

(H) Western blot analyses of EVs and cell lysate.