Figure 4.

EVs enrich for unique RNA signatures that are different from their cancer cell of origin

(A) Capillary electrophoresis analyses of small RNA from donor cells (22RV1) and their EVs.

(B) Principal component analysis (PCA) of the RNA between cells and EVs shows distinct RNA in EVs.

(C) Volcano plot showing differential enrichment of RNA between Cells and EVs.

(D) Hallmark cancer pathways identified in EV and cellular RNA.

(E) Two biological runs yielded a high correlation of cell versus cell RNAseq.

(F) Two biological runs yielded a high correlation between EVs vs EVs RNAseq.

(G) Comparison between donor cells versus their EVs RNA does not yield a high correlation.

(H) A normalized gene expression of six different cell lines for donor cells versus their EVs RNAseq. In all studies, EVs enriched for distinct RNA, which were predominantly different in their cell of origin.