REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD81	Abcam	PRID: ab239687
Calnexin	Abcam	PRID: ab22595
Gold conjugated secondary antibody	Abcam	PRID: ab105285
Flotillin (anti-Flot-1)	Abcam	PRID: ab133497
Biological samples
Serum samples from prostate cancer patients	Icahn School of Medicine at Mount Sinai; Department of Urology	IRB, GCO # 06-0996, 14-0318
Human prostate cancer cell line 22RV1	American Type Culture Collection	ATCC CRL-2505
Chemicals, peptides, and recombinant proteins
RPMI 1640 cell culture medium	GIBCO
Bovine serum albumin in phosphate buffer saline	Sigma Aldrich
3% Glutaraldehyde	Sigma Aldrich
Osmium tetraoxide	Sigma Aldrich
Acid-phenol	Invitrogen	Cat# 4478545
Chloroform	Invitrogen	Cat# 4478545
Lysis Buffer (2% SDS/1X protease inhibitor/0.1M Ambic)	Invitrogen	Cat# 4478545
Critical commercial assays
Total EV RNA and Protein Isolation Kit	Invitrogen	Cat# 4478545
Deposited data
Raw and normalized 22RV1 gene and protein counts	This manuscript	GSE123736
Human reference genome Ensembl GRCh38.p13	Ensembl	https://useast.ensembl.org/Homo_sapiens/Info/Annotation
Human breast cancer cell lines EV and cell proteomic counts	Hurwitz et al., 2016	PMID: 27894104
Human colon cancer cell lines EV and cell gene counts	Hinger et al., 2018	PMID: 30332650
Human normal and cancer-associated fibroblasts EV and cell gene counts	Herrera et al., 2018	PMID: 30075793
Human reticulocyte derived EV protein counts	Diaz-Varela et al., 2018	PMID: 30232403
Human glioblastoma cell derived 7.6572mmEV gene counts	Wei et al., 2017	PMID: 29074968
Software and algorithms
QIAGEN Ingenuity Pathway Analysis	QIAGEN	https://digitalinsights.qiagen.com/products-overview/discovery-insights-portfolio/analysis-and-visualization/qiagen-ipa/?cmpid=QDI_GA_IPA&gclid=CjwKCAjwgr6TBhAGEiwA3aVuIS7UzgcUf8gTC8ad6uXMnBwZEeEb_lhG88SStmpLMASvAUl0tbI9tBoCJeAQAvD_BwE
FeatureCounts read summarization function	Subread	http://subread.sourceforge.net/
bowtie aligner (version 2.5.4b)	bowtie	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
R version 4.1.1	R Core Team	https://www.R-project.org/.
Proteome Discoverer software (version 2.1)	Thermo fisher scientific	https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html
Qlucore Omics Explorer software	Qlucore	https://qlucore.com/
Code availability	Github	https://github.com/chentytina/22RV1_EV