Figure 2.

Blocking axonal Na⁺ influx with TTX barely affects mitochondrial motility parameters altered due to oxidative stress. (a) Representative original images of all different experimental conditions: axon incubated with aCSF alone contained multiple moving mitochondria that c5over larger distances in the axon; 100 μM H₂O₂ induced a strong reduction of motile mitochondria; axons simultaneously incubated with 100 μM H₂O₂ and 100 nM TTX or 1 μM TTX contained more motile mitochondria, covering longer distances. (b) Percentage of moving mitochondria per axon. Nodes of Ranvier are marked with a red “i”. ^∗p ≤ 0.05, ^∗∗∗∗p ≤ 0.0001. The error bars represent the standard error of mean; n = 6 animals and 22 roots; untreated 7 roots, H₂O₂ 6 roots, H₂O₂+100 nM TTX 4 roots, and H₂O₂+1 μM TTX 5 roots.