Fig. 3.

Notch3 knockdown decreases tumorigenesis with downregulation of GPX4 and PRDX6 in vivo. (A) Representative xenografted tumor images. (B) Volumes of tumors formed in BALB/c athymic nude mice. Data were reported as mean ± SEM, and statistical analysis was performed via two‐tailed Student's t tests. (C) Weights of tumors formed in BALB/c athymic nude mice. Data were reported as mean ± SEM, and statistical analysis was performed via two‐tailed Student's t tests. (D–F) Representative immunohistochemical staining of Notch3, GPX4, and PRDX6. All sections were counter‐stained with hematoxylin. Scale bar, 100 μm. (G) Western blot analysis of GPX4 and PRDX6 expressions of tumors. Left, representative western blot. Right, histogram of protein expressions. Data were reported as mean ± SEM, and statistical analysis was performed via two‐way ANOVA; *P < 0.05 and **P < 0.01.