Subject	Cell Biology
Specific subject area	Screening of chemicals with human thyroid receptor (hTR)-antagonist activity by a yeast two-hybrid assay.
Type of data	Figure and Raw data
How the data were acquired	Bioassay: Yeast two-hybrid assay. Instrument: Luminescencer JNR AB2100; Atto Corp., Tokyo, Japan. (Luminescence was read on a 96-well plate luminometer)
Data format	Analyzed (Figure), raw (Excel)
Description of data collection	The hTR-antagonist activities of 691 chemicals were evaluated by a yeast two-hybrid assay using Saccharomyces cerevisiae Y190 introduced hTRα and coactivator. The stock solutions of the 691 compounds were prepared with dimethyl sulfoxide (DMSO) and stored at −30°C in darkness.
Data source location	• Institution: Health and Environmental Risk Division, National Institute for Environmental Studies (NIES) • City/Town/Region: Tsukuba city, Ibaraki prefecture • Country: Japan
Data accessibility	Raw data was deposited at Mendeley data. Repository name: The hTR-antagonist activities of 691 chemicals Data identification number: 10.17632/546fbt7g8m.1 Direct URL to data: https://data.mendeley.com/datasets/546fbt7g8m/1