FIG. 1.

Saccharification of cellulose by CbhA and CbhB. One percent (wt/vol) cellulose was digested overnight at 30°C with enzyme solutions of partially purified CbhA or CbhB. Fifty-microliter portions of twofold-diluted heat-inactivated (5 min, 100°C) samples were analyzed by high-performance anion-exchange chromatography on a Dionex system with a Carbopac PA-100 column and by pulsed amperometrical detection by using a gradient of 0.05 to 0.90 M NaOH suitable for glucose oligosaccharide separation. The CMC sample represents the non-enzyme-treated substrate as a negative control. Standards (stand.) used were d-glucose and cellobiose.