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. 2022 May 21;15:100300. doi: 10.1016/j.mtbio.2022.100300

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 8 — The recruitment and infiltration of neutrophils and macrophages to the wound sites and elastin-based hydrogels at different timepoints. (A) HE staining of wound tissue after treatment of elastin-based hydrogels for 3, 7 and 14 days. The enlarged images of GAE-50 clearly showed the polymorphonuclear neutrophils, which were pointed out by the red-circles. Scale bar: 200 μm (Black), 50 μm (White); (B) Immunohistochemical staining of macrophages in the wound tissue after treatment of elastin-based hydrogels for 3, 7 and 14 days. F4/80: pan-macrophages; CD86: M1 macrophages; CD206: M2 macrophages. GAE-0, GAE-50, GAE-100: Wounds treated by GelMA hydrogels, composite hydrogels containing 50% and 100% modified elastin, respectively. Scale bar: 200 μm.