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. 2022 May 18;13:833286. doi: 10.3389/fimmu.2022.833286

Figure 3.

Figure 3

Comparison of genes differentially expressed between monocytes. (A) mRNA was purified from monocytes co-cultured in four different conditions: RBC 0: MØ + 100% fresh RBC; RBC 21: MØ + 100% stored RBC for 21 days at 4°; Mix 1: MØ + 80% fresh RBC + 20% stored RBC for 21 days at 4°C and Mix 2: MØ + 90% fresh RBC + 10% stored RBC for 21 days at 4°C. Data are expressed as mean relative gene expression in arbitrary units. (B) HO-1, (C) Spic, (D) ferritin, (E) CX3CR1, (F) CD206, and (G) IL-6 mRNA expression in monocytes was measured by TLDA in four different conditions: RBC 0: MØ + 100% fresh RBC; RBC 21: MØ + 100% stored RBC for 21 days at 4°; Mix 1: MØ + 80% fresh RBC + 20% stored RBC for 21 days at 4°C, and Mix 2: MØ + 90% fresh RBC + 10% stored RBC for 21 days at 4°C. Results are expressed as 3 - 6 different experiments. Data are expressed as mean ± standard error (SEM). *Represents p < 0.05.