Fig. 10.

Effect of aging on the germination performance of Arabidopsis (Col- WT) and Os02g0817500 ectopic expression lines ‘OE-1’ and ‘OE-2’. A Semi-quantitative RT-PCR analysis of the expression of Os02g0817500 in Arabidopsis leaves. Atactin was used as a positive control. The original data can be viewed from Additional file 5: Fig. S6. B Germination rates of seeds before and after artificial aging. Data represent mean ± SD (n = 3). Significant differences between WT and transgenic samples were determined using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001). C Phenotypes of 5-day-old seedlings produced from unaged seeds (Left) and 10-day-old seedlings produced from artificially aged seeds (Right). Seedlings were grown on the half-strength Murashige and Skoog (MS) medium