Table 1.
Variable | Aa-WT | A-MIM164c | A-OE164c | Bb-WT | B-MIM164c | B-OE164c | Total | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Number | % | Number | % | Number | % | Number | % | Number | % | Number | % | Number | % | |
Raw reads | 23,290,765 | n.a.c | 39,475,861 | n.a. | 105,051,201 | n.a. | 11,574,728 | n.a. | 23,358,523 | n.a. | 75,229,379 | n.a. | 277,980,457 | n.a. |
Reads< 15 nt in length after removing the 3′-adaptor sequences | 115,963 | 0.5 | 150,392 | 0.38 | 438,675 | 0.42 | 52,118 | 0.45 | 114,625 | 0.49 | 291,424 | 0.39 | 1,163,197 | 0.42 |
Mappable reads | 23,174,802 | 99.5 | 39,325,469 | 99.62 | 104,612,526 | 99.58 | 11,522,610 | 99.55 | 23,243,898 | 99.51 | 74,937,955 | 99.61 | 276,817,260 | 99.58 |
Transcript mapped reads | 13,086,100 | 56.19 | 19,232,591 | 48.72 | 49,082,507 | 46.72 | 6,072,389 | 52.46 | 12,433,643 | 53.23 | 35,928,730 | 47.76 | 135,835,960 | 48.8 |
Unique raw reads | 5,910,027 | n.a. | 7,499,373 | n.a. | 14,920,325 | n.a. | 4,409,611 | n.a. | 6,992,505 | n.a. | 14,893,430 | n.a. | 35,326,716 | n.a. |
Unique reads < 15 nt in length after removing three adaptor sequences | 31,617 | 0.53 | 41,124 | 0.55 | 77,208 | 0.52 | 24,911 | 0.56 | 37,938 | 0.54 | 80,857 | 0.54 | 181,550 | 0.51 |
Unique mappable reads | 5,878,410 | 99.47 | 7,458,249 | 99.45 | 14,843,117 | 99.48 | 4,384,700 | 99.44 | 69,54,567 | 99.46 | 14,812,573 | 99.46 | 35,145,166 | 99.49 |
Unique transcript mapped reads | 3,675,827 | 62.2 | 4,501,572 | 60.03 | 7,887,621 | 52.86 | 2,797,531 | 63.44 | 3,961,237 | 56.65 | 8,498,489 | 57.06 | 17,031,820 | 48.21 |
No. of input transcripts | 42,387 | n.a. | 42,387 | n.a. | 42,387 | n.a. | 42,387 | n.a. | 42,387 | n.a. | 42,387 | n.a. | 42,387 | n.a. |
No. of covered transcripts | 33,806 | 79.76 | 33,227 | 78.39 | 36,332 | 85.71 | 34,687 | 81.83 | 35,095 | 82.8 | 38,044 | 89.75 | 39,538 | 93.28 |
aA indicates unaged seeds
bB indicates artificially aged seeds
cn.a. indicates not applicable