Table 3.
PLSCR1 interactions with viral proteins
| Virus | Protein | Study model | methods | Effects of PLSCR1 interaction | Pro/anti | References |
|---|---|---|---|---|---|---|
| HBV | HBx |
- HEK-293 - HepG2 - Huh7 |
- Yeast two-hybrid screening - GST pull-down assay - CO-IP |
Induces HBx ubiquitination and proteasome degradation | Anti virus | [32] |
| HIV | TAT |
- COS-1 - MOLT/HIV |
- Pull-down assay - CO-IP |
Inhibits Tat functions by reducing its nuclear localization | Anti virus | [28] |
| SLPI |
- CD4+ HPB-ALL - Jurkat |
- Yeast two-hybrid screening - GST pull-down assay - CO-IP - ELISA interaction test |
Perturbs the virus entry process by modulating CD4-SLPI interaction | Anti virus | [85] | |
| HTLV1 | TAX | COS-1 |
- Pull-down assay - CO-IP |
Reduces the cytoplasmic distribution of TAX and its homodimerization | Anti virus | [27] |
| IAV | NP |
- A549 - HEK-293 T - THP-1 - U251 |
- Yeast two-hybrid screening - CO-IP |
Inhibits the nuclear import of NP/vRNP thus limiting viral replication | Anti virus | [29] |
| EBV | BZLF1 |
- HEK-293 - COS-1 - HeLa - A431 - MCF-7 - SW480 - C666-1 - BJAB - B958 - Namalwa - P3HR1 - Daudi |
- Pulldown assay - CO-IP |
Represses BZLF1-dependent lytic gene expression | Anti virus | [87] |
| HCMV |
CREB CBP IE2 |
- HEL - OUMS-36 T-3 - HEK-293 |
- Pull-down assay - CO-IP |
Inhibits CREB functions and CREB-IE2, CBP-IE2 complexes, resulting in the repression of HCMV replication | Anti virus | [89] |
|
HSV1 HSV2 |
Glycoprotein L |
- CaSki - K2/E6E7 - Vero - HaCAT |
- PLA - CO-IP |
Favors AKT translocation to the outer leaflet of plasma membrane followed by glycoprotein B binding and viral entry | Pro virus | [90] |
| HCV |
E1 E2 OCLN |
- HEK-293 T - Huh7.5.1 |
- Yeast two-hybrid screening - Pull-down assay - CO-IP |
Interacts with HCV envelope proteins and favors viral entry | Pro virus | [26] |
HBV Hepatitis B virus, HBx HBV encoded X protein, HCC hepatocellular carcinoma, CO-IP Co-immunoprecipitation, HIV-1 human immunodeficiency virus type-1, SLPI Secretory leukocyte protease inhibitor, HTLV-1 Human T-cell leukemia virus type-1, IAV influenza A virus, NP nucleoprotein, vRNP viral ribonucleoprotein, EBV Epstein-Barr virus, HSV Herpes simplex virus, PLA Proximity ligation assay, HCV Hepatitis C virus, OCLN occluding, HCMV Human cytomegalovirus, CREB cAMP-responsive element-binding protein, IE2 HCMV immediate early protein 2, CBP CREB-binding protein