FIGURE 2.

Impact of the Ser37Tyr mutation observed in the DDAC-adapted IT-BIO-DC-139 strain on DNA binding. Carbon atoms are shown in green for the parental strain (IT-FOX-FE-63) (A) and in blue for the DDAC-adapted strain IT-BIO-DC-139 (B). The calculated hydrogen bonds are indicated by a yellow dotted line. Only the scaffold is shown for the double-stranded DNA (dsDNA) molecule. The dsDNA is docked in the parental strain; the modification of the residue 37 alters the conformation of the protein at the DNA-binding site, leading to a reduction of affinity for the DNA strands (affinity score of –82.9 compared with –104.2 from the fepR model of the parental strain). (DDAC, didecyldimethylammonium chloride).