TABLE 1.
MPN estimation using BIOLOG plates
| DL or estimated cell density | Observed (expected) no.a of positive wells with the following inoculum and substrate:
|
||
|---|---|---|---|
|
E. coli
|
WTP (xylose) | ||
| Xylose | Asparagine | ||
| 1 | 84 (84.0) | 84 (84.0) | 84 (84.0) |
| 2 | 84 (84.0) | 84 (84.0) | 83 (83.8) |
| 3 | 84 (84.0) | 84 (84.0) | 39 (37.9) |
| 4 | 83 (84.0) | 84 (84.0) | 4 (4.9) |
| 5 | 84 (84.0) | 84 (84.0) | 1 (0.5) |
| 6 | 84 (84.0) | 84 (84.0) | 0 (0.1) |
| 7 | 84 (84.0) | 84 (84.0) | |
| 8 | 76 (75.1) | 69 (70.1) | |
| 9 | 15 (16.9) | 14 (13.8) | |
| 10 | 3 (1.9) | 4 (1.5) | |
| 11 | 1 (0.2) | 0 (0.2) | |
| Estimated cell density at DL = 0b (no. of cells/ml) | 1.5 × 109 | 1.2 × 109 | 4.0 × 103 |
a
The observed number of positive wells of 84 replicates and the expected number (shown in parentheses) are shown.
b
The cell densities at DL = 0 were determined so that the expected numbers would show the best agreement with the observations.