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. 2022 May 18;13:855858. doi: 10.3389/fpls.2022.855858

Table 2.

Degree of leaves visibly wilted, plant growth index (PGI), relative chlorophyll content [Soil Plant Analysis Development (SPAD) value], water content of leaves and stems, stem water potential (ψstem), and leaf curling index of Shepherdia ×utahensis at eight substrate volumetric water content treatments (θt).

Water contenty
θt (m3·m−3) Leaves wilted (1–5 scale)z PGIy SPAD Leaf (%) Stem (%) ψstem (MPa) Leaf curling indexx
0.40 4.7 abv 33.4 a 58.9 a 59.2 a 62.7 a −0.82 ab 0.04 bc
0.35 4.8 a 33.2 a 58.2 a 62.6 a 63.6 a −0.65 a 0.05 bc
0.30 4.7 ab 36.7 a 58.3 a 59.7 a 60.4 a −0.90 ab 0.02 c
0.25 3.7 bc 23.1 b 56.1 ab 58.1 a 58.5 ab −1.95 bc 0.06 bc
0.20 3.4 cd 22.6 b 52.1 ab 57.1 a 58.7 ab −1.45 b 0.03 bc
0.15 2.2 de 17.2 b 49.0 abc 51.1 a 49.9 b −1.98 c 0.10 abc
0.10 1.9 e 18.3 b 43.4 bc 51.2 a 52.4 b −1.97 bc 0.11 ab
0.05 1.0 e 17.6 b 34.9 c 23.3 b 36.8 c −5.76w 0.17 a
Linear **u NS NS NS NS NS NS
Quadratic NS NS NS *** *** NS NS
Cubic **** **** **** **** **** * ****
z

1 = over 65% of the leaves wilted; 2 = 35–65% of the leaves wilted; 3 = up to 35% of the leaves wilted; 4 = less than 10% of the leaves wilted; and 5 = plant was fully turgid (Zollinger et al., 2006).

y

Plant growth index = [(height + length + width)/3], while water content of leaves and stems was calculated using the equation: [fresh weight (FW) − dry weight (DW)/FW x 100% (Zhou et al., 2021).

x

Leaf curling index was determined using the equation: [distance between the margins of flattened leaf (Dmax)-distance between the margins of curling leaf (Di)]/Dmax (Nilsen, 1987).

W

Only two data were recorded due to high plant mortality.

v

Means with same lowercase letters within a column are not significantly different among treatments by Tukey–Kramer method with a significance level specified at 0.05.

u

NS, *, **, ***, **** Nonsignificant, significant at p ≤ 0.05, 0.01, 0.001, or 0.0001, respectively.