TABLE 2.
Consensus matrix of tests for identification of Enterococcus spp.a
| Test or characteristic | E. asini | E. avium | E. casseliflavus | E. durans | E. faecalis | E. faecium | E. gallinarum | E. hirae | E. malodoratus | E. mundtii | E. pseudoavium | E. solitarius | E. raffinosus | E. cecorum | E. dispar | E. saccharolyticus | E. sulfureus | E. columbae | E. flavescens | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Metabolism with: | ||||||||||||||||||||
| N-Acetylglucosamine | + | + | + | + | + | + | + | + | + | + | + | + | + | + | ND | ND | + | + | + | |
| Adonitol | − | + | − | − | − | − | − | − | + | − | + | − | d | − | − | − | − | − | − | |
| Amygdalin | + | + | + | + | + | + | + | + | + | + | + | + | ND | + | ND | ND | + | V | + | |
| l-Arabinose | − | + | + | − | − | + | + | − | − | + | − | − | + | − | − | − | − | + | + | |
| d-Arabitol | − | + | − | − | − | − | − | − | + | − | − | ND | + | d | − | + | − | V | − | |
| l-Arabitol | − | d | − | − | − | − | − | − | + | − | + | − | + | − | − | − | − | − | − | |
| Arbutin | + | + | + | + | − | + | + | + | + | + | ND | + | ND | + | ND | ND | + | + | + | |
| Cellobiose | + | + | + | + | + | + | + | + | + | + | + | + | ND | + | ND | ND | + | + | + | |
| Dextrin | ND | (+) | + | ND | + | V | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Dulcitol | − | d | − | − | − | − | − | − | d | − | − | ND | − | − | − | − | − | − | − | |
| Erythritol | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | ND | ND | ND | − | − | − | |
| d-Fructose | + | + | + | + | + | + | + | + | + | + | ND | + | ND | + | ND | ND | + | + | + | |
| d-Fucose | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | − | ND | ND | − | − | − | |
| l-Fucose | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | − | ND | ND | − | − | − | |
| Galactose | + | + | + | + | + | + | + | + | + | + | + | + | ND | + | ND | ND | + | + | + | |
| Gluconate | − | (+) | + | − | (+) | V | + | − | + | − | ND | + | ND | d | − | − | + | − | + | |
| d-Glucose | + | + | + | + | + | + | + | + | + | + | ND | ND | ND | + | ND | ND | + | + | ND | |
| Glycerol | − | d | V | − | + | d | d | d | d | d | − | + | + | − | + | − | − | − | − | |
| Glycogen | − | − | − | − | − | − | d | − | − | (−) | − | − | − | − | − | ND | − | − | − | |
| Inulin | − | d | (+) | − | − | − | d | − | d | d | − | − | − | + | − | + | − | + | + | |
| 2-Ketogluconate | − | + | − | − | V | − | − | − | + | − | + | − | + | V | + | + | + | (−) | − | |
| 5-Ketogluconate | − | V | − | − | − | − | − | − | − | − | ND | − | ND | V | ND | ND | ND | − | − | |
| Lactose | + | + | + | + | + | + | + | + | + | + | + | − | + | + | + | ND | + | (+) | + | |
| d-Lyxose | − | + | − | − | − | − | − | − | − | − | ND | − | ND | − | − | − | − | − | − | |
| Maltose | + | + | + | + | + | + | + | + | + | + | + | + | ND | + | ND | ND | + | + | + | |
| Mannitol | − | + | + | − | + | + | + | − | + | + | + | + | + | d | − | + | − | + | + | |
| d-Mannose | + | + | + | + | + | + | + | + | + | + | ND | + | + | + | ND | ND | + | + | + | |
| Melibiose | − | d | + | d | − | (+) | + | + | + | + | − | (−) | + | + | d | + | + | + | ND | |
| Melezitose | − | + | d | − | (+) | − | d | − | − | d | − | + | + | d | − | + | + | (−) | − | |
| Methyl-α-d-glucopyranoside | (−) | + | + | − | (−) | − | + | − | d | − | + | + | + | d | + | + | + | + | + | |
| Methyl-α-d-mannopyranoside | − | V | V | − | (−) | − | (−) | − | + | (+) | ND | − | ND | − | ND | ND | + | − | − | |
| Methyl xyloside | − | − | − | − | − | − | − | − | − | − | ND | ND | ND | − | ND | ND | ND | − | − | |
| d-Raffinose | − | − | d | − | − | d | + | + | d | + | (+) | − | − | + | + | + | + | + | + | + |
| Rhamnose | + | + | (+) | − | v | − | − | − | + | + | − | − | + | − | ND | ND | − | (−) | + | |
| Ribose | − | + | + | + | + | + | + | + | + | + | + | − | + | + | + | ND | + | + | − | |
| Salicin | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
| Sorbitol | − | + | d | − | + | − | d | − | d | d | + | d | + | d | − | + | − | + | − | |
| Sorbose | − | + | − | − | − | − | − | − | + | − | + | ND | + | − | − | − | ND | − | − | |
| Starch | (+) | − | V | d | d | d | d | (+) | ND | d | − | − | V | + | − | ND | ND | + | − | |
| Sucrose | − | (+) | + | d | + | + | + | + | + | + | d | + | + | + | + | ND | + | + | + | |
| d-Tagatose | − | + | − | − | + | − | + | (−) | d | − | − | + | + | − | ND | ND | − | V | − | |
| Trehalose | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | ND | + | + | + | |
| d-Turanose | − | d | V | − | − | − | + | (−) | V | − | ND | + | ND | − | ND | ND | ND | (−) | − | |
| l-Xylose | − | d | − | − | − | − | (−) | − | V | − | ND | − | ND | ND | ND | ND | − | ND | − | |
| d-Xylose | + | d | + | − | V | d | + | − | V | + | ND | − | ND | − | − | − | − | + | + | |
| Xylitol | − | + | − | − | − | − | − | − | + | − | ND | − | ND | − | − | − | − | (−) | − | |
| Growth at: | ||||||||||||||||||||
| 4°C | ND | − | ND | ND | − | + | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| 10°C | V | d | + | + | + | + | + | + | + | + | + | (+) | − | − | + | + | + | − | (−) | |
| 45°C | V | + | (+) | + | + | + | + | + | − | + | + | + | + | + | − | + | − | ND | (+) | |
| 50°C | ND | − | − | − | − | V | − | − | − | − | ND | ND | ND | ND | − | − | ND | ND | ND | |
| pH 9.6 | ND | + | + | + | + | + | + | + | ND | + | + | ND | + | ND | ND | ND | ND | ND | ND | |
| Growth in: | ||||||||||||||||||||
| 6.5% NaCl | − | d | + | + | + | + | + | + | + | + | d | + | + | − | + | + | + | − | ND | |
| 0.1% Methylene blue milk | ND | − | ND | + | + | + | V | ND | ND | ND | ND | ND | ND | V | ND | ND | ND | ND | ND | |
| 0.04% Tellurite | ND | − | d | − | + | − | d | − | − | d | − | − | − | ND | ND | ND | ND | ND | ND | |
| 0.01% Tetrazolium | ND | ND | ND | − | + | − | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Survival at 60°C for: | ||||||||||||||||||||
| 15 min | ND | + | ND | ND | + | + | (+) | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| 30 min | + | + | ND | ND | + | + | d | ND | ND | ND | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| 1 h | ND | (−) | ND | ND | + | + | − | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Gelatin liquefaction | ND | − | − | − | d | − | − | − | − | − | ND | ND | ND | − | ND | ND | ND | ND | ND | |
| H2S production | ND | d | − | − | − | − | − | ND | + | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | |
| Alpha hemolysis | ND | + | + | V | V | d | d | d | − | − | d | − | − | d | ND | ND | ND | ND | − | |
| Beta hemolysis | ND | − | − | V | V | d | d | − | − | − | − | − | − | d | ND | ND | ND | ND | − | |
| Lancefield group D | + | + | + | (+) | + | V | + | V | + | + | − | + | ND | − | − | − | − | − | + | |
| Motility | − | − | + | − | − | − | + | − | − | − | − | − | − | − | − | − | − | ND | + | |
| Voges-Proskauer | ND | d | d | + | + | + | d | + | d | + | d | d | d | d | ND | − | ND | + | + | |
| Yellow pigment | − | − | + | − | − | − | − | − | − | + | − | − | − | − | − | − | + | − | + | |
| Alkaline phosphatase | − | − | ND | − | d | (−) | − | − | − | − | − | − | − | + | − | − | − | + | − | |
| Arginine dihydrolase | − | − | d | + | + | + | + | + | − | + | − | + | − | − | + | − | − | − | + | |
| Catalase | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | |
| Esculin hydrolysis | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
| α-Galactosidase | − | − | + | − | − | d | + | + | + | (+) | d | + | d | d | + | + | + | + | + | |
| β-Galactosidase | − | d | + | d | d | + | + | (+) | + | + | d | − | − | d | + | ND | + | V | + | |
| β-Glucoronidase | − | − | − | − | − | − | d | − | − | − | − | − | − | + | − | ND | − | − | − | |
| Hippurate hydrolysis | + | d | − | V | d | d | d | d | d | − | d | d | d | d | V | − | − | − | − | |
| Leucine arylamidase | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | |
| Pyrrolidonyl aminopeptidase | + | + | + | + | + | + | + | + | + | + | + | + | + | − | + | − | + | − | + |
a
+, 90% or more of the strains of isolates are positive; (+), 75 to 89% are positive; V, 26 to 74% are positive; (−), 11 to 25% are positive; −, 10 or less are positive; ND, no data; d, discrepancies among reference studies.