Table 2. Interface surface areas due to crystal contacts.
Interface surface areas between the MagaFab chains (VL and VH), the peptide (Pep) and symmetry-related chains (marked with an asterisk) calculated with the PISA server (Krissinel & Henrick, 2007 ▸). The interface surface areas between the main peptide (Pep) and main MagaFab chains (VL and VH) are included as a reference. Numerous residues are involved in these contacts, mainly through hydrogen-bond and hydrophobic interactions.
| Chain 1 | Chain 2 | Interface surface area (Å2) |
|---|---|---|
| VL* (chain C) | VL (chain A) | 262.5 |
| VH (chain B) | 0 | |
| Pep (chain E) | 14.2 | |
| VH* (chain D) | VL (chain A) | 31.6 |
| VH (chain B) | 0 | |
| Pep (chain E) | 187.1 | |
| Pep* (chain F) | VL (chain A) | 78.0/71.4† |
| VH (chain B) | 210.2 | |
| Pep (chain E) | 352.0 | |
| Pep (chain E) | VL (chain A) | 338.2 |
| VH (chain B) | 356.0 |
†
The second value is an interaction with a crystal contact VL (Asn2Pep*–Ser207VLneigh).