FIG. 5.

Induction of mnpl transcription in the presence of OH · . (A to D) Northern blot analysis of total RNA from samples of washed mycelium from glucose-ammonium medium incubated for 120 min in the presence of 500 μM menadione and 100 μM Fe³⁺ generating OH · (B), 500 μM menadione generating H₂O₂ (C), 100 μM Fe³⁺ (D), and the corresponding control without the addition of the above-mentioned compounds (A), with mnpl2 cDNA and ribosomal DNA from D. melanogaster (control) (all samples were incubated in 20 mM sodium tartrate, pH 5). (E) Time course of normalized mnpl mRNA levels (as percentages of maximal transcript levels in peptone medium after normalization to same rRNA in each sample) corresponding to B (○), C (□), and A (▵). The H₂O₂ level is also shown (dashed line).