Human milk SARS-CoV-2-specific antibody levels and neutralizing activity persist after Holder pasteurization (n = 24). The median level of SARS-CoV-2-specific IgM, IgG, and IgA in human milk collected 1 month after vaccination were compared pre- and postpasteurization (A, B, and C, respectively). Neutralization activity was compared pre- and postpasteurization (D). Wilcoxon matched-pairs signed-rank tests were used for statistical analysis. Error bars indicate interquartile range.