Fig. 3.

Effect of GTF2B on tumor cell phenotype of GH3 cell line. A. Proliferation analysis of tumor cell with different transfection by MTT assay. OD value of control group at 0 h was normalized as 100% and all relative cell proliferation value at different time points are shown in column diagram above; the table below displays the relative inhibitory rate vs. control group at each time point. B. Transwell array was used to assess GH3 cell invasiveness with various treatments (original magnification, ×200). C. ELISA was performed to test GH secretion of GH3 cell with various treatments. D. Western blot analysis of Ki-67, ZAC-1, E-cadherin, MMP2 and MMP9 expression in GH3 cell line with various treatments. Each condition was tested in triplicate. The two-tailed Student’s t-tests were used. Bar represents mean ± SD, *P < .05, **P < .01, ***P < .001, and ****P < .0001.