Fig. 5.

Knockdown of CD55 suppresses NB development in vivo. (A) Representative Western blotting shows that suppression of CD55 downregulates p-JNK, OCT4, and NANOG, whereas upregulates p15 in SKN-BE(2)C cells; (B) 2 million cells were injected subcutaneously into NOD/SCID mice. Mice were sacrificed about two months later. 10 out of 12 mice injected with control SKN-BE(2)C cells generated tumors; however, only 5 out of 12 mice injected with CD55-knockdown cells formed tumors (n = 5); (C) Representative H&E staining and histological characterization of SKN-BE(2) tumors derived from either control cells or CD55-knockdown cells. Note that NB derived from CD55-knockdown cells present with more differentiated phenotype (H&E), yellow arrowheads indicate ganglia or neuropil like structure in the tumors. Immunohistochemical staining shows the expression of PCNA, Cyclin D1, c-Caspase 3, NANOG, OCT4, and p-JNK, scale bar = 50 µm; (D) Quantification data of (C).