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. 2022 Mar 8;10:843687. doi: 10.3389/fcell.2022.843687

FIGURE 1.

FIGURE 1

Experimental setup of Connexin43 nanogold labeling in murine cardiac sections. (A) Thick cross sections from murine control hearts preserved in 4% paraformaldehyde, stored in a 24 wells plate. Sections are sectioned with a vibratome and of 200 µm thickness. (B) FluoroNanogold labeling of adult murine cardiac sections. Left panel: FluoroNanogold-546 labeled Connexin43 visualization by light microscopy. Scalebar: 10 µm. Zoom-in at indicated region presented in the panel below, arrowheads depict the intercalated disc (ID). Scalebar: 5 µm. Right panel: Nanogold visualization after silver enhancement of Connexin43 by light microscopy. Pictures in both frames are from the same cardiac section. Scalebar: 50 µm. Zoom-in at indicated region presented in the panel below, arrowheads depict the intercalated disc (ID). Scalebar: 10 µm. (C) Examples of flat embedded cardiac sections after nanogold labeling. In the left panel the entire cross sections are presented. On the right, two representative examples are shown from dissections of left or right ventricular samples. RV: Right ventricle, LV: Left ventricle.