. 2022 Jun 1;17(6):e0269090. doi: 10.1371/journal.pone.0269090

Table 1. Summary of the ID, age group, predominant pathological process detected histologically the CNS, and pooled histoscore averages of the protein marker immunoreactivity for the VCN and IC of the dolphins of this study (0—No immunoreactivity; 3—Very intense immunoreactivity).

ID	Age/ age group	Categories			Predominant pathological proccess	Histoscore averages for the VCN and IC
ID	Age/ age group	A	B	C	Predominant pathological proccess	Apaf-1	DGK-ζ	Bcl-2	Aβ	NF200
146	Adult	N	N	Y	Cell death and degeneration	1.42^nc; 1.71^ac	2.63ⁿⁿl; 0^nc	0^all	1.5ⁿⁿ; 2.15^nc	2.69^nc
20	30 y	N	N	O	Cell death and degeneration	0.43^nc; 0.88^ac	1.01^nnl; 0.7^nc	1.09ⁿc; 0.6^ac	0.9ⁿⁿ; 1.07^nc; 2.0^ac	2.39^nc
89	5 y	N	N	Y	Cell death and degeneration	0.7^nc; 1.57^ac	2.16^nnl; 0.47^nc	0.6^nc; 0.55^ac	2.4ⁿⁿ; 1.08^nc; 1.55^ac	2.69^nc
139	30 y	N	N	O	Cell death and degeneration	0.65^nc; 0.9^ac	1.45^nnl; 0.68^nc	0^all	0.93ⁿⁿ; 0.8^nc; 2.1^ac	2.98^nc
159	40 y	N	N	O	Inflammation; Cell death and degeneration	1.0^nc; 1.46^ac	0.3^nnl; 0.84^nc	0^all	0.6ⁿⁿ; 1.0^nc, 2.0^ac	2.94^nc
319	Old	N	N	O	Inflammation; Hypoxia	1.45^nc; 2.13^ac	0.15^nnl; 0.72^nc	0.1^nc; 0.59^ac	1.92ⁿⁿ; 1.22^nc; 1.7^ac	2.61^nc
344	Sub-adult	N	P	Y	Hemodynamic disorder	0.95^nc; 1.76^ac	0.99^nnl; 0.74^nc	0^all	2.17ⁿⁿ; 1.0^nc; 1.9^ac	2.78^nc
192	Adult	N	N	Y	None detected	0.8^nc; 1.64^ac	1.79^nnl; 0.52^nc	0^nc; 0.75^ac	1.63ⁿⁿ; 1.48^nc; 1.9^ac	2.69^nc
196	Old	P	P	O	Inflammation (associated with T. gondii)	0.92^nc; 1.75^ac	1.5^nnl; 0.62^nc	0.6^nc; 0.6^ac	1.39ⁿⁿ; 1.48^nc; 1.85^ac	2.85^nc
95	Adult	P	P	O	Hemodynamic disorder; Inflammation	0.87^nc; 1.98^ac	1.3^nnl; 0.89^nc	1.0^nc; 0.4^ac	1.9ⁿⁿ;1.23^nc; 2.05^ac	2.59^nc
107	9 y	P	N	Y	Inflammation; Cell death	1.04^nc; 1.89^ac	2.38^nnl; 0.41^nc	0^all	1.78ⁿⁿ; 1.4^nc; 1.85^ac	2.7^nc
133	>30 y	P	P	O	Inflammation; Cell death	0.8^nc; 1.79^ac	1.72^nnl; 0.9^nc	0^all	1.77ⁿⁿ; 1.13^nc; 2.0^ac	2.91^nc
142	Old	P	P	O	Inflammation (associated with T. gondii)	0.6^nc; 1.32^ac	0.48^nnl; 0.27^nc	0^all	0.8ⁿⁿ; 0.67^nc; 2.0^ac	2.47^nc
165	Old	P	P	O	Inflammation (associated with T. gondii)	0.37^nc; 1.99^ac	0.34^nnl; 0.94^nc	0^all	2.05ⁿⁿ; 1.12^nc; 1.8^ac	2.81^nc
201	Old	P	P	O	Inflammation (associated with DMV)	0.86^nc; 2.05^ac	0.9^nnl; 1.01^nc	0^all	1.77ⁿⁿ; 1.1^nc; 2.02^ac	2.96^nc
203	Old	N	P	O	Cell death and degeneration; Hypoxia	1.11^nc; 1.03^ac	0.05^nnl; 1.21^nc	0^all	0.0ⁿⁿ; 1.38^nc; 1.75^ac	1.78^nc
114	9 d	C	C	C	Cell death and degeneration	1.59^nc; 1.88^ac	2.07^nnl; 0.72^nc	1.09^nc; 0.82^ac	2.34ⁿⁿ; 1.3^nc; 1.45^ac	2.27^nc
144	9 d	C	C	C	Cell death	1.8^nc; 1.84^ac	1.53^nnl; 0.99^nc	0.95^nc; 0.2^ac	2.76ⁿⁿ; 1.32^nc; 1.65^ac	2.71^nc
145	9 d	C	C	C	Cell death	1.54^nc; 1.73^ac	2.62^nnl; 0.02^nc	1.17^nc; 0.42^ac	2.7ⁿⁿ; 1.31^nc; 1.6^ac	2.79^nc
343	Calf	C	C	C	Cell death	1.79^nc; 1.4^ac	0.97^nnl; 1.05^nc	0^all	2.78ⁿⁿ; 1.0^nc; 1.4^ac	2.92^nc

Columns A, B and C apply to the grouping of the animals according to microscopic changes in hematoxylin-eosin (HE)-stained slides (A), independent IHC slide analysis (B), and age (C). N—adults without visible lesions in HE-slides or observed aberration in the IHC slides; P—adults with observed microscopic lesions/aberrant IHC-patterns, Y—young (<30 y.o.) adult, O—old (≥30 y.o.) adult. C—cal. M—male; F—female. Subcellular compartments: ac—astrocyte cytoplasm; all—all cell types and compartments; an—astrocyte nuclei; nc—neuronal cytoplasm; nn—neuronal nuclei; nnl—neuronal nucleoli.