KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
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| ||
| Rabbit polyclonal cytokeratin CK8 | Abcam | Cat# ab59400; RRID: AB_942041 |
| Recombinant Alexa Flour 488 anti-Cytokeratin 8 | Abcam | Cat# 192467: RRID: AB_2864346 |
| Recombinant Alexa Flour 647 anti-Cytokeratin 5 | Abcam | Cat# 193895; RRID: AB_2728796 |
| Rabbit monoclonal E-Cadherin (24E10) | Cell Signaling technology | Cat# 3195T |
| Donkey anti-rabbit IgG secondary antibody Alexa 488 conjugate. | Thermo-Fisher Scientific | Cat# A21206; RRID: AB_2535792 |
| NeuN | Millipore | Cat# MAB377; RRID: AB_2298772 |
| GFAP | Agilent DAKO | Cat# Z033429-2; RRID: AB_10013382 |
| CD31 | R&D systems | Cat# AF3608; RRID: AB_2169833 |
| Ki67 | Spring Bioscience | Cat# M3062; RRID: AB_11219741 |
| RFP | Rockland | Cat# 600-401-379; RRID: AB_2209751 |
| Phospho-S6 | Cell Signaling technology | Cat# 5364; RRID: AB_10694233 |
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| Bacterial and virus strains | ||
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| ||
| Luc/Cre lentiviral plasmid | Cho et al., 2014, 2015 | Addgene plasmid 20905 |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Dapi | Thermo Fisher Scientific | Cat# D1306; RRID: AB_2629482 |
| Normal Donkey Serum | Sigma | D9663-10ML; RRID: AB_2810235 |
| Paraformaldehyde | Electron Microscopy Sciences | Cat# 19210 |
| Sodium Periodate | Sigma | Cat# S1878 |
| Sodium borohydrate | Sigma | Cat# 452882 |
| Sucrose | Sigma | Cat# S8501 |
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| Deposited data | ||
|
| ||
| Raw and analyzed data | This paper | Tables S1, S2, S3, S4, and S5 |
|
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| Experimental models: Organisms/strains | ||
|
| ||
| C57BL/6J | Jackson Laboratory | Cat # 000664 |
| PtenloxP/loxP Trp53loxP/loxP | Cho et al., 2014 | PMID: 24444712 |
| RosaCAG-tdTomato-loxP-stop-loxP | Jackson Laboratory | Cat # 007909 |
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| ||
| Oligonucleotides | ||
|
| ||
| PtenloxP/loxP, F: 5′-TGTTTTTGACCAATTAAAGTAGGCTGTG-3′ | This paper | N/A |
| PtenloxP/loxP, R:5′AAAAGTTCCCCTGCTGATGATTTGT-3′ | This paper | N/A |
| Trp53loxP/loxP, F: 5′-CACAAAAACAGGTTAAACCCAG-3′ | This paper | N/A |
| Trp53loxP/loxP, R: 5′-AGCACATAGGAGGCAGAGAC-3′ | This paper | N/A |
| Wt tdTomato, F: 5′-AAGGGAGCTGCAGTGGAG TA-3′ | This paper | N/A |
| Wt tdTomato, R: 5′-CCGAAAATCTGTGGGAAGTC-3′ | This paper | N/A |
| Mut tdTomato F: 5′-GGCATTAAAGCAGCGTATCC-3′ | This paper | N/A |
| Mut tdTomato R: 5′-CTGTTCCTGTACGGCATG 3′ | This paper | N/A |
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| ||
| Software and algorithms | ||
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| FIJI software | NIH | https://imagej.net/software/fiji, RRID: SCR_00228 |
| MATLAB software | MathWorks | https://www.mathworks.com/products/matlab.html?s_tid=hp_products_matlab; RRID: SCR_001622 |
| GraphPad Prism 9 software | GraphPad Software | https://www.graphpad.com/; RRID: SCR_002798 |
| Imaris software | Version 9.2 Bitplane | http://www.bitplane.com/imaris/imaris; RRID: SCR_007370 |
| Code for STPT | (Kim et al., 2015) | https://www.ncbi.nlm.nih.gov/pubmed/25558063 |