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. 2022 May 16;132(10):e154611. doi: 10.1172/JCI154611

Figure 1. Skeletal muscle atrophy triggered by immobilization is prevented in mice with KLF15 deficiency in skeletal muscle.

Figure 1

(A) Ratio of gastrocnemius or soleus muscle mass in both hind limbs to body mass for control mice or mice subjected to bilateral hind limb immobilization (IM) with a cast for 3 days (n = 18 mice). (B and C) Quantitative RT-PCR analysis of Klf15 mRNA (B) and of atrophy-related gene expression (C) in gastrocnemius of mice as in A (n = 8 mice). (D) Ratio of muscle mass to body mass for WT or M-KLF15KO mice subjected to cast immobilization for 3 days or for corresponding control (Cont) mice (n = 8 mice). (E–G) Hematoxylin-eosin staining (E) for determination of muscle fiber area (F) and the distribution of muscle fiber area (G) in soleus of mice as in D (n = 8 mice). The area of 800 fibers pooled from 4 mice was measured and averaged for each condition in F. Scale bar: 50 μm. (H) Quantitative RT-PCR analysis of atrophy-related gene expression in gastrocnemius of mice as in D (n = 6 mice). Quantitative data are mean ± SEM (A–D, G, and H) or medians (F). *P < 0.05, **P < 0.01 by unpaired Student’s t test (A–C) or 2-way ANOVA with Bonferroni’s post hoc test (D and F–H). NS, not significant.