Figure 6.

YAP regulates RGMa-induced integrity of the BBB. (A) Immunofluorescence showed that YAP was expressed in CD31⁺ endothelial cell both in EAE mice and HBMECs. Scale bar: 50 µm (brain cortex and HBMECs) and 25 µm (spinal cord). (B–D) YAP expression gradually decreased with the progression of EAE. (n = 4, error bar: SEM; ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, ^**** p < 0.0001, one-way ANOVA with Bonferroni). (E, F) YAP expression was significantly diminished when overexpressing RGMa in vitro (n = 3, ^** p < 0.01, t-test). (G, H) The YAP expression level was ameliorated when blocking BMPR II (n = 3, ^* p < 0.05, t-test). (I–K) By overexpressing YAP by LPA 20 µM for 2 h after overexpressing RGMa, both ZO-1 and claudin-5 were significantly increased (n = 3, ^** p < 0.01, t-test).