Skip to main content
. 2022 May 25;2022:5477024. doi: 10.1155/2022/5477024

Figure 4.

Figure 4

miR-664a-5p regulates biological function in VSMCs through targeting OPA1. (a) qRT-PCR analysis of miR-664a-5p mRNA expression in atherosclerotic plaques of patients and healthy people. (b) Annexin V-FITC/propidium iodide staining and FACS quantification of the number of apoptotic cells in HA-VSMC after transfection of miR-664a-5p mimic. (c, d) MitoTracker staining of mitochondrial fission in VSMC after transfection of miR-664a mimic or Punisher. (e, f) MitoTracker staining of mitochondrial fission in VSMCs after transfection of miR-664a inhibitor with or without H2O2 stimulation. (g, h) Evaluation of ROS production after transfection of miR-664a-5p mimic or inhibitor. (i) Measurement of mitochondrial membrane potential after transfection of miR-664a-5p inhibitor or Punisher. (j) Evaluation of ATP production after transfection of miR-664a-5p inhibitor or Punisher. All values are the average of at least three biological replicates, and data shown are the mean ± SD. Scale bars: 20 μm. ∗p < 0.05; ∗∗p < 0.01.