FIG. 2.

Microinjection of various ¹⁴C-labeled metabolites into individual sections of agarose-embedded guts of Cubitermes spp. (C. umbratus and C. orthognathus) incubated under air or H₂ atmosphere. (A) Distribution of radioactivity between guts and agarose at the end of the incubation period. Incubation times were 45 min, except for series 6 (20 min) and 7 and 8 (15 min). Each bar represents the mean of three to four injections into separate guts, except for PEG (six to eight injections per section). Error bars indicate standard deviations of the total recovery. (B) Product formation rates from labeled substrates. (C) Efflux rates of labeled metabolites into the surrounding agarose. In the case of the unidentified product (series 6), the rates were calculated for a C₁ compound. Bar definition in panel C is the same as in panel B. For definitions of the sections, see Fig. 1 in the companion paper (33).