Extended Data Fig. 6. Mtb encodes diverse mechanisms of intrinsic resistance to ribosome-targeting antibiotics.

a, Ethambutol checkerboard assays to quantify drug-drug interactions. Dose-response curves (mean ± SEM, n = 3 biological replicates) are shown for each drug in the absence (DMSO) or presence of 0.25X MIC₈₀ of EMB. Fractional inhibitory concentration index (FICI) values listed represent the lowest value obtained from each checkerboard assay. b, Phylogenetic tree of antibiotic resistance (ARE) ABC-F proteins from the indicated species. Figure adapted from⁴⁴ Bootstrap values (500 replicates) are indicated at each node. c, Growth curves for the linezolid-associated hit genes and control strains shown in Fig. 3d. Curves (mean ± SEM, n = 3 biological replicates) are derived from the vehicle control samples of the MIC assay. NT = non-targeting; KD = knockdown. d, Dose-response curves (mean ± SEM, n = 3 biological replicates) were measured for CRISPRi knockdown strains targeting smpB and clpP2 in wild-type H37Rv. e, Growth curves for the strains shown in panel (d). Curves (mean ± SEM, n = 3 biological replicates) are derived from the vehicle control samples of the MIC assay. f, Dose-response curves (mean ± SEM, n = 6 biological replicates) of WT H37Rv and an isogenic rplC-Cys154Arg mutant. g, Dose-response curves (mean ± SEM, n = 3 biological replicates) for the indicated CRISPRi strains.