Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 4;606(7912):153–159. doi: 10.1038/s41586-022-04686-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a, Coronal sections containing sexually dimorphic brain areas used for ERα CUT&RUN. MPOA, medial pre-optic area; BNSTp, posterior bed nucleus of the stria terminalis; MeAp, posterior medial amygdala. b, Line plots (top) and heatmaps (bottom) of mean IgG and ERα CUT&RUN (C&R) CPM ±1 kb around E2-induced ERα CUT&RUN peaks (DiffBind edgeR, P_adj < 0.1). The heatmaps are sorted by E2 ERα CUT&RUN signal. Colour scale is counts per million (CPM). Veh, vehicle. c, Cross-tissue ERα comparison, showing the proportion of ERα peaks detected specifically in brain. d, Top Gene Ontology biological process terms associated with genes nearest to brain-specific or shared (≥4 other tissues) ERα CUT&RUN peaks (clusterProfiler, P_adj < 0.1). e, Combined sex E2 versus vehicle RNA-seq in BNSTp Esr1+ cells; light grey and red dots (DESeq2, P_adj < 0.1), dark grey and red dots (DESeq2, P < 0.01), purple dots (validated by in situ hybridization (ISH)). FC, fold change. Positive FC is E2-upregulated, negative FC is E2-downregulated. f, Images (left panels) and quantitative analysis (right panels) of ISH for select genes induced by E2 in both sexes. Boxplot centre, median; box boundaries, first and third quartiles; whiskers, 1.5 × IQR from boundaries. Two-way analysis of variance: Brinp2 P = 0.0373, Rcn1 P = 0.0307, Enah P = 0.0003, Tle3 P = 0.0001; n = 4 per condition; scale bar, 200 µm. g, MA plot of E2-regulated ATAC–seq peaks in BNSTp Esr1+ cells; red dots are E2-open peaks (DiffBind edgeR, log₂[FC] > 1, P_adj < 0.05), grey dots are E2-close peaks (DiffBind edgeR, log₂[FC] < −1, P_adj < 0.05). h, Example ERα peaks at E2-induced genes. Top left number is the y-axis range in CPM. Shaded band indicates peak region.

Source Data