TABLE 7.
Comparison of the species-specific primer method with the classical culture method when the same samples were used
| Sample | Taxon | Results of:
|
|
|---|---|---|---|
| PCR methoda | Culture method (log CFU/g) | ||
| AD-1 | B. adolescentis | + | 9.5 |
| B. catenulatum group | + | 8.9 | |
| B. dentium | + | NDb | |
| AD-2c | Bifidobacterium species | − | ND |
| AD-3 | B. adolescentis | + | 9.4 |
| B. catenulatum group | + | 9.6 | |
| B. longum | + | 8.3 | |
| B. dentium | + | ND | |
| AD-4 | B. bifidum | + | 9.8 |
| B. breve | + | ND | |
| B. catenulatum group | + | 10.0 | |
| B. longum | + | ND | |
| AD-5 | B. adolescentis | + | ND |
| B. catenulatum group | + | 9.4 | |
| AD-6 | B. adolescentis | + | ND |
| B. catenulatum group | + | 8.6 | |
| AD-7 | B. catenulatum group | + | 8.9 |
| B. longum | + | ND | |
| AD-8 | B. catenulatum group | + | 10.2 |
| AD-9 | B. adolescentis | + | ND |
| B. bifidum | + | 8.9 | |
| B. catenulatum group | + | 9.8 | |
| B. longum | + | ND | |
| AD-10 | B. adolescentis | + | ND |
| B. bifidum | + | 9.6 | |
| B. catenulatum group | + | ND | |
| B. longum | + | 10.3 | |
a
+, positive; −, negative.
b
ND, not detected.
c
No Bifidobacterium species was detected by either the PCR method or the culture method.