Fig. 10. Neuroprotective effect of compound 11 on CM from a microglia/neuroblastoma co-culture model.

BV-2 microglial cells were pretreated with or without compound 11 (1–25 μM) and then stimulated with LPS (0.2 µg/mL) for 6 h. The culture media was discarded, and fresh medium was added. After 24 h of incubation, the culture supernatants were collected and then added to HT-22 neuronal cells as described in the Methods. After co-culture for 24 h, cell viability was determined using an MTT reagent. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the LPS-alone group.