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. 2021 Aug 24;43(6):1508–1520. doi: 10.1038/s41401-021-00753-x

Fig. 5. Effects of compounds 5, 9, 11, and 12 on the production of NO and the mRNA expression of pro-inflammatory factors in glial cells.

Fig. 5

Cells were pretreated with 25 μM compounds 5, 9, 11, and 12 for 30 min prior to LPS or LPS + INF-γ stimulation. a, b After 24 h of LPS (0.2 µg/mL) or LPS+INF-γ (50 U) stimulation, the levels of NO in the supernatants of primary microglial cells (a) and primary astrocytes (b) were determined by Griess reagent. c–e RT-qPCR analysis of TNF-α (c), IL-1β (d), and IL-6 (e) mRNA levels after LPS stimulation for 6 h. *P < 0.05, **P < 0.01, ***P < 0.001 compared to the LPS-alone group.