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. 2022 Jun 1;11(1):30. doi: 10.1038/s41389-022-00406-6

Fig. 1. ACBI1 depletes SWI/SNF subunits BRG1 and PBRM1.

Fig. 1

a G401 cells were treated with 250 nM ACBI1 for the indicated times and protein lysates were probed for expression of BRG1, pBAF-specific subunits PBRM1, ARID2, and BRD7, and MYC. DMSO control is matched to longest treatment. GAPDH is used as a loading control. b G401 cells were treated as in a for 24 h and protein lysates probed for the indicated proteins. GAPDH is used as a loading control. c G401 cells were plated with 250 nM ACBI1 or DMSO control and cell growth monitored over 24 h (n = 5 biological replicates, error bars are standard error). d G401 cells in c were allowed to grow for 4 days in the presence of ACBI1 or DMSO control and cells counted at this timepoint (n = 5 biological replicates, error bars are standard error, **P = 0.004 using unpaired t-test, two-tailed between DMSO and ACBI1). e Cell cycle analysis of G401 cells treated with 250 nM ACBI1 or DMSO control for 4 days as determined by flow cytometry (n = 4 biological replicates, error bars are standard error, **P = 0.0015, *P = 0.0117 using unpaired t-test, two-tailed).