Table 2.

Comparative summary of the Experiment-2 in vitro inhibition kinetics¹ of GH5-tCel5A1, the heat-treated GH5-tCel5A1 and GH5-p4818Cel5_2A under the gastric acidic pH of 3.5 and pepsin (274 U/mL) obtained through the Eadie-Hofstee linear regression analyses.

Item	Inhibition kinetic parameter estimates
	$I_{\mathit{max}}$ 2		$I_{\mathit{min}}$ 3	$I_c$ 4	${\mathit{IC}}_{50}$ 5
Cellulase activity (nmol/mg protein·min)
GH5-tCel5A1	2.70 $\pm 3.78$		17.43 $\pm 1.46$	20.13 $\pm 3.78$	0.70 $\pm 0.09$
Heated GH5-tCel5A1	36.56 $\pm 16.04$		38.48 $\pm 6.44$	74.05 $\pm 16.04$	1.05 $\pm 0.12$
GH5-p4818Cel5_2A	57.14 $\pm 3.69$		26.42 $\pm 2.66$	83.55 $\pm 3.69$	0.83 $\pm 0.07$
Relative cellulase activity (% -20of the control)6
GH5-tCel5A1	13.42 $\pm 19.72$		86.58 $\pm 7.23$	100.00 $\pm 19.72$	0.70 $\pm 0.09$
Heated GH5-tCel5A1	48.72 $\pm 21.38$		51.28 $\pm 8.59$	100.00 $\pm 21.38$	1.05 $\pm 0.12$
GH5-p4818Cel5_2A	68.38 $\pm 4.42$		31.62 $\pm 3.19$	100.00 $\pm 4.42$	0.83 $\pm 0.07$

¹Values are parameter estimates or means $\pm$ SE (n = 72) obtained through the Eadie-Hofstee regression linear analyses, representing the inhibition kinetic parameter estimates obtained from the three gastric acidic pH 3.5 and pepsin (274 U/mL) inhibition experiments with 4 replicates per time point in each experiment, as shown in Figs. 9, 10, 11.

² $I_{\mathit{max}}$ is the maximal magnitude of inhibition in the cellulase activity (nmol/mg protein·min or % of the control, I_c; means $\pm$ SE, n = 12) calculated according to Eq. (2).

³ $I_{\mathit{min}}$ is the minimal residual cellulase activity (nmol/mg protein·min or % of the control I_c; parameter estimate $\pm$ SE, n = 72) under the combined gastric acidic pH 3.5 and pepsin (274 U/mL) inhibition conditions obtained from Eq. (1).

⁴ $I_c$ is the mean cellulase activity of the control group (nmol/mg protein·min or % of the control I_c; means $\pm$ SE, n = 12).

⁵ ${\mathit{IC}}_{50}$ is the incubation time (h, parameter estimate $\pm$ SE, n = 72) required to reach the half maximal inhibition of the cellulase activity.

⁶Relative cellulase activity calculated as percentage (%, means $\pm$ SE, n = 12) of the control group ($I_c$) measured at 0-min exposure to the combined gastric pH-3.5 and pepsin (274 U/mL) incubation.