Table 3.

Comparative summary of the Experiment-3 in vitro inhibition kinetics¹ of GH5-tCel5A1, the heat-treated GH5-tCel5A1 and GH5-p4818Cel5_2A under the intestinal trypsin (74 U/mL) condition obtained through the Eadie-Hofstee linear regression analyses.

Item	Inhibition kinetic parameter estimates
	$I_{\mathit{max}}$ 2		$I_{\mathit{min}}$ 3	$I_{\mathrm{c}}$ 4	${\mathit{IC}}_{50}$ 5
Cellulase activity (nmol/mg protein·min)
GH5-tCel5A1	12.48 $\pm 2.41$		0.68 $\pm 0.25$	13.16 $\pm 2.41$	1.64 $\pm 0.09$
Heated GH5-tCel5A1	3.26 $\pm 1.47$		1.92 $\pm 0.35$	5.17 $\pm 1.47$	1.32 $\pm 0.09$
GH5-p4818Cel5_2A	69.98 $\pm 2.56$		9.76 $\pm 1.16$	79.74 $\pm 2.56$	1.20 $\pm 0.05$
Relative cellulase activity (% of the control)6
GH5-tCel5A1	$94.83\pm 18.29$		5.$17\pm 1.90$	100.00 $\pm 18.29$	1.64 $\pm 0.09$
Heated GH5-tCel5A1	87.39 $\pm 9.68$		12.60 $\pm 2.32$	100.00 $\pm 9.68$	1.32 $\pm 0.09$
GH5-p4818Cel5_2A	87.76 $\pm 3.21$		12.24 $\pm 1.45$	100.00 $\pm 3.21$	1.20 $\pm 0.05$

¹Values are parameter estimates or means $\pm$ SE (n = 72) obtained through the Eadie-Hofstee regression linear analyses, representing the inhibition kinetic parameter estimates obtained from the three intestinal trypsin (74 U/mL) inhibition experiments with 4 replicates per time point in each experiment, as shown in Figs. 12, 13, 14.

² $I_{\mathit{max}}$ is the maximal magnitude of inhibition in the cellulase activity (nmol/mg protein·min or % of the control, I_c; means $\pm$ SE, n = 12) calculated according to Eq. (2).

³ $I_{\mathit{min}}$ is the minimal residual cellulase activity (nmol/mg protein·min or % of the control, parameter estimate $\pm$ SE, n = 72) under the intestinal trypsin (74 U/mL) inhibition condition obtained from Eq. (1).

⁴ $I_c$ is the mean cellulase activity of the control group (nmol/mg protein·min or % of the control I_c; means $\pm$ SE, n = 12).

⁵ ${\mathit{IC}}_{50}$ is the incubation time (h, parameter estimate $\pm$ SE, n = 72) required to reach the half maximal inhibition of the cellulase activity.

⁶Relative cellulase activity calculated as percentage (%, means $\pm$ SE, n = 12) of the control group ($I_c$) measured at 0-min exposure to the intestinal trypsin (74 U/mL) incubation.