Table 4.

Comparative summary of the Experiment-3 in vitro inhibition kinetics¹ of GH5-tCel5A1, the heat-treated GH5-tCel5A1 and GH5-p4818Cel5_2A under the intestinal Chymotrypsin (20 U/mL) condition obtained through the Eadie-Hofstee linear regression analyses.

	Inhibition kinetic parameter estimates
Item	$I_{\mathit{max}}$ 2		$I_{\mathit{min}}$ 3	$I_c$ 4	${\mathit{IC}}_{50}$ 5
Cellulase activity (nmol/mg protein·min)
GH5-tCel5A1	7.21 $\pm 0.68$		0.37 $\pm 0.17$	7.57 $\pm 0.68$	1.59 $\pm 0.12$
Heated GH5-tCel5A1	11.84 $\pm 1.41$		3.34 $\pm 0.42$	15.18 $\pm 1.41$	0.99 $\pm 0.14$
GH5-p4818Cel5_2A	108.51 $\pm 2.69$		1.12 $\pm 0.17$	109.63 $\pm 2.69$	1.19 $\pm 0.09$
Relative cellulase activity (% of the control)6
GH5-tCel5A1	95.18 $\pm 8.94$		4.82 $\pm 2.27$	100.00 $\pm 8.94$	1.59 $\pm 0.12$
Heated GH5-tCel5A1	78.01 $\pm 9.26$		21.98 $\pm 2.79$	100.00 $\pm 9.26$	1.00 $\pm 0.14$
GH5-p4818Cel5_2A	98.98 $\pm 2.46$		1.02 $\pm 0.16$	100.00 $\pm 2.46$	1.20 $\pm 0.09$

¹Values are parameter estimates or means $\pm$ SE (n = 72) obtained through the Eadie-Hofstee regression linear analyses, representing the inhibition kinetic parameter estimates obtained from the three chymotrypsin (20 U/mL) inhibition experiments with 4 replicates per time point in each experiment, as shown in Figs. 15, 16, 17.

² $I_{\mathit{max}}$ is the maximal magnitude of inhibition in the cellulase activity (nmol/mg protein·min or % of the control, means $\pm$ SE, n = 12) calculated according to Eq. (2).

³ $I_{\mathit{min}}$ is the minimal residual cellulase activity (nmol/mg protein·min or % of the control I_c; parameter estimate $\pm$ SE, n = 72) under the intestinal chymotrypsin (20 U/mL) inhibition condition obtained from Eq. (1).

⁴ $I_c$ is the mean cellulase activity of the control group (nmol/mg protein·min or % of the control, means $\pm$ SE, n = 12).

⁵ ${\mathit{IC}}_{50}$ is the incubation time (h, parameter estimate $\pm$ SE, n = 72) required to reach the half maximal inhibition of the cellulase activity.

⁶Relative cellulase activity calculated as percentage (%, means $\pm$ SE, n = 12) of the control group (I_c) measured at 0-min exposure to the intestinal chymotrypsin (20 U/mL) incubation.