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. 2021 Sep 3;43(6):1360–1371. doi: 10.1038/s41401-021-00759-5

Fig. 6. Metformin ameliorates SCI-induced blockade of autophagic flux in microglial cells.

Fig. 6

a, b Western blotting and quantification analysis of p-AMPK and p-mTOR in the spinal cord from each group at 14 d after SCI (n = 3). c–e Western blotting and quantification analysis of p62 and ATG7 in the spinal cord from each group at 14 d after SCI (n = 3). f Co-staining of LAMP1 (green) and LC3II (red) in posterior horn area of spinal cords from each group at 14 d post SCI (n = 5). Scale bar = 25 μm. g Co-staining of IBa-1 (green) and LC3II (red) in posterior horn area of spinal cord from each group at 14 d post SCI (n = 5). Scale bar = 25 μm. #P < 0.05, ##P < 0.01 vs. Sham group, *P < 0.05, **P < 0.01, ***P < 0.001 vs. SCI group. SCI spinal cord injury, Met metformin.