Fig. 2. Variation in isolate collection location, identity, and timing across all sampling.

Error bars indicate standard error of intensive care unit (ICU) rooms. ** indicates generalized linear mixed-modeling (GLMM) p-value <0.01. a In-room and bathroom sink drains have significantly more isolates per collection than other surface locations in both the old and new ICU buildings (n = 566 surface isolates). Locations in light gray were not collected in old ICU. b Genus of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF) species identification of all collected isolates in both the new and old ICU. Other Surface includes all other surfaces that are not in-room or bathroom sink drain. c Variation in number of isolates collected per bathroom or in-room sink drain sample collection by building (excludes fecal and communal samples, n = 429). d Variation in number of isolates per other surface sample collection by building (excludes sink drain, fecal, and communal samples, n = 137). e Variation in number of isolates per bathroom or in-room sink drain sample collection for all time points, n = 429. f Variation in number of isolates collected per other surface sample collection across all time points (excludes sink drain, fecal, and communal samples, n = 137). Gray bars indicate weeks with incomplete sampling of surfaces. BP before patient and staff move-in.