Fig. 3. Identification of specific markers for Tas cells.

a Volcano plots showing differentially expressed genes between CD4⁺ T-shared and T-specific cells, CD8⁺ T-shared and T-specific cells, and CD4⁺ T-shared cells and CD4⁺ Treg cells. Blue dots represent significantly upregulated genes in CD4⁺ T-specific, CD8⁺ T-specific and CD4⁺ Treg cells respectively (|log2(FC)| > 0.58, P < 0.05). b Heatmap showing the expression of selected genes in CD4⁺ T-specific, CD8⁺ T-specific and CD4⁺ Treg cells, CD4⁺ T-shared and CD8⁺ T-shared cells (right panel); c Box plot showing the expression of CXCL13 in CD4⁺ (top panel) and CD8⁺ T cell clusters (bottom panel). d Line chart showing the frequency of each TCR in the four tissues. The top 20 TCRs of CD4⁺CXCL13⁺, CD4⁺CXCL13⁻, CD8⁺CXCL13⁺, and CD8⁺CXCL13⁻ T cells were presented. Each line represents a TCR. e The correlation between the percents of CXCL13⁺ T cells in tumor T cells and the TMB of each tumor. f The correlation between the median expression of CXCL13 and TMB of each cancer type.